Lab Protocols | Hematopia

sykes lab protocols

Isolation of bone marrow cells from murine femurs and tibias

Bone Marrow Spin Flush

Cell cycle analysis

Propidium Iodide Staining

Removal of platleets and leukocytes from whole blood

Red Blood Cell Isolation

Timing pregnancies for embryonic sample collection

Timed Mouse Pregnancies

Transduction of cells via centrifugation

Spinfection

Production and titer of lentivirus or retrovirus

Transfection

Transplantation of bone marrow for the assessment of stem cell defects

Competitive Bone Marrow Transplant

Production and immortalization of murine bone marrow stromal cells

Bone Marrow Stroma