TEMPI
ER-Hoxb8
Sykes Lab
Hematopoiesis
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Isolation of bone marrow cells from murine femurs and tibias
Bone Marrow Spin Flush
Cell cycle analysis
Propidium Iodide Staining
Removal of platleets and leukocytes from whole blood
Red Blood Cell Isolation
Timing pregnancies for embryonic sample collection
Timed Mouse Pregnancies
Transduction of cells via centrifugation
Spinfection
Production and titer of lentivirus or retrovirus
Transfection
Transplantation of bone marrow for the assessment of stem cell defects
Competitive Bone Marrow Transplant
Production and immortalization of murine bone marrow stromal cells
Bone Marrow Stroma