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sykes lab protocols

Lab-Curated Protocols

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Isolation of bone marrow cells from murine femurs and tibias

Bone Marrow Spin Flush

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Cell cycle analysis

Propidium Iodide Staining

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Removal of platleets and leukocytes from whole blood

Red Blood Cell Isolation

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Timing pregnancies for embryonic sample collection

Timed Mouse Pregnancies

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Transduction of cells via centrifugation

Spinfection

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Production and titer of lentivirus or retrovirus

Transfection

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Transplantation of bone marrow for the assessment of stem cell defects

Competitive Bone Marrow Transplant

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Production and immortalization of murine bone marrow stromal cells

Bone Marrow Stroma

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